Description: Bromothymol Blue (BTB) is a pH indicator (pKa approx. Relevance. PubChem Substance ID 24892083. For this reason it can be electrostatically immobilized on the surface on anion-exchanger resin like Amberlite IRA 401. Assume a cuvet with a 14.45 mm diameter was used. Chloroform showed the highest affinity for bromothymol blue, followed by methylene chloride, ethylene dichloride, benzene, toluene, and carbon tetrachloride. 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the mic... SDS. Ensure that the solutions are mixed well. 1 decade ago. It shows that pH=pKa where [ln-]= [Hln] or hwere ( [ln-]/ [Hln])=1 or where log ( [ln-]/ [Hln])=0. Using bromothymol blue you wouldn't be able to distinquish between anacid with pH 4 from an acid with pH 5. Solutions of different concentration of the indicator were used. Acid - Base indicators are either weak acids or weak bases so they exist as molecules in equilibrium with their ions. Place three small drops of bromothymol blue solution into each of the 5 cuvettes. 2. In this experiment, spectrophotometry is employed to measure the pKa of bromothymol blue, an acid-base indicator. Empirical Formula (Hill Notation) C 27 H 27 Br 2 NaO 5 S . pH = pKa - log ( [HIn] / [In-] ) … (2) Into each of the cuvettes place 3 mL of the solutions of the various pH values (hydrochloric acid, the buffer solutions with pH values 6.0, 7.0 and 8.0, and sodium hydroxide solution). The indicator (HIn) is a monoprotic acid and we can represent its dissociation as follows: HIn → H+ + In- … (1) The equilibrium expression for such dissociation can be written as. Explain why the acid - base indicator bromothymol blue turns yellow when added to dilute HCl but blue when added to dilute NaOH. EC Number 252-169-7. There is a linear relationship between log ( [ln-]/ [Hln]) and pH. Molar absorptivity ___ (You will be using this value in your experiment.) 2 Answers. NACRES NA.47 Favorite Answer. Computational methods, such as SQUAD (84) 16 and SPECFIT/32, and some new methods Spectrophotometric determination of the pKa of Bromothymol Blue. Indicator: pH Range: pKa: Acid Form: Base Form: Methy Violet: 0.0 - 1.6: 0.8: Yellow: Blue: Thymol Blue: 1.2 - 2.8: 1.6: Red: Yellow: Methyl Yellow: 29. The pKa can be determined from the spectrophotometric data using different methods. Answer Save. ,IC10113005EA,IC10113025EA. Supplier: MP Biomedicals. Bromothymol blue, Powder ACS. The pKa′ value was determined using a method similar to that of Reilley and Swayer. Bromothymol Blue sodium salt powder Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt CAS Number 34722-90-2. Beilstein/REAXYS Number 6887926 . Determination of the pK_a of Bromothymol Blue Use the following graph to determine the molar absorptivity of Bromothymol blue (BTB)? MDL number MFCD00077263. [10] [11] describe multiwavelength analysis and nonlinear least squares regres-sion methods. 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